Name:              Mohammad Ghazanfari                     

Date of Birth:      Sep / 22 / 1976

Place of Birth:     Khomein, Iran

E-mail:                 mohghazanfari@yahoo.com   &  mohghazanfari@gmail.com

URL:                    http://www.zistamooz.com

Education

University:  

·         Research assistant in National Institute for Genetic Engineering and Biotechnology (NIGEB),

         Tehran- Iran, (2000 – 2005).

·         M.sc., Biology  (Cellular & Molecular Science ), Faculty of Sciences, Tehran university- Iran,

         (1999-2001).

·         B.Sc , Biology  -Tarbiyat moalem university- Tehran-Iran , (1995-1999)

  High school:

·   Diploma of Natural Sciences, Mostafa Khomeini High school, Khomein, Iran. (1994).

Research Experience

·         Research Assistant in National Institute for Genetic Engineering and    Biotechnology (NIGEB), Tehran, Iran. (2000-2005).

·         Research Assistant in Research Center for Gastroenterology and Liver Diseases. (RCGLD), Tehran, Iran.      (2005-2007).

M.Sc. Thesis title

                Study of Methylentetrahydrofolate  reductase (MTHFR) Gene mutation as a cause of

            maternal Down Syndrome in Iranian cases.

Laboratory Skills

• DNA Extraction

• RNA extraction

• PCR

• Digestion with restriction enzymes

• Gel electrophoresis, including protein(SDS-PAGE) and nucleic acids (Poly acrylamide and Agarose)

• western and Southern  blotting

• Plasmid extraction

• RT-PCR

• Certification of the workshop on good laboratory practice(GLP), September   2000, NRCGEB       

        And our following activities are:

• PCR-SSCP

• Cell Culture

• ELISA

• Primer designing

• Cloning and Expression of gene in E.Coli

•  

Computer skills

Word, Processing, PowerPoint, Excel, Photoshop, Video Studio, Internet and some genetics soft wares.

Publications

• S.Aleyasin, F.Jahanshad, S.Mohammad ganji, M.Ghazanfari,  N.Atabak, Study of molecular etiology of Down Syndrome in Iran. Second Conference of Medical Genetics, Ardebil, Iran. Septembr, 13-14, 2000.

• Aleyasin A, Jahanshad F, Mohammad ganji S, Ghazanfari M,  Atabak N. Study of methylentetrahydrofolate reductase  Gene  mutation as a maternal risk factor of Down Syndrome in Iran. 50^th annual meeting of American Society of Human Genetics, Pennsylvania Convention Center, Philadelphia, Pennsylvania, USA, October 3-7, 2000.

• S.Aleyasin, M. Ghazanfari (2001), Folath pathway gene alteration in patients with leber optic nerve syndrome (LHON). 10^th  international congress of human genetics, May 15-19, 2001, Vienna-Austria.

• A. Aleyasin, M. Ghazanfar; Folic acid gene Alteration as maternal cause of  Down syndrome. 18^th ICCC (International Congress of Clinical Chemistry and Laboratory Medicine. Kyoto, Japan. October 20-25, 2002.

• M. Ghazanfari, A. Aleyasin ; Study of folic Acid pathway genes alteration as maternal cause of Down Syndrome. European human Genetics conference, Birmangam, England. May 3-6, 2003.

• M. Ghazanfari, A. Aleyasin; Higher frequency of methylentetrahydrofolate reductase (MTHFR) gene alteration and degeneration of optic nerve. The first scientific meeting of the Asian society for Mitochondrial research and medicine. Seoul, Korea. February 5-6, 2003.

·         Ahmad Aleyasin PhD, Shahla Mohammad-Ganji MSc, Mohammad Ghazanfari MSc, Faezeh Jahanshad MD^; Parental origin of meiotic error of the extra chromosome 21 as indicated by short tandem repeat (STR) polymorphisms in Down syndrome. ARCHIVES OF IRANIAN MEDICINE.  April 2004, Volume 7, Number 2. 118-121. Iran.

• A.Aleyasin, S.Mohammad ganji, M.Ghazanfari, F. Jahanshad. Application of molecular DNA markers (STRs) in molecular diagnosis of Down Syndrome in Iran. Journal of Science. Islamic Republic of Iran. Journal of Sciences, Islamic Republic of Iran 15(2): 103-108 (2004). University of Tehran, ISSN 1016-1104.

• Ahmad Aleyasin, Mohammad Ghazanfari, Massoud Houshmand. of Medical Genetic Group. National Institute for Genetic Engineering and Biotechnology Folate pathway gene alteration and etiology of Leber hereditary optic neuropathy (LHON). 4th National Biotechnology Congress of Iran. 2005.

• Ghazanfary M., Motevaly F., Momeni S K, Roohvand F. Salmanzadeh S., Habibi M., Zali M R: A comparative study for identification and genotyping of Adenoviruses by three molecular methods based on Hexon, AV-RNA and Fiber genes. Proceedings of 4^th national congress of biotechnology, Kerman-Iran, August 2005, (In Persian with English abstract).

• M  Ghazanfari, S. Salmanzadeh-Ahrabi, F. Jafari, Dr. M  Zali, Prevalence and typing of adenoviruses in stool of Iranian children with acute diarrhea-A comparative study by molecular and serological assays. F  Motevali, Dr. F Rohvand, 16th EuropeanCongress of Clinical Microbiology and Infectious Diseases (ECCMID), Oxford, UK. 01.04.2006 - 04.04.2006

• F. Roohvand, F. Motevali, M. Ghazanfari, S. Salmanzadeh-Ahrabi, F. Jafari, M. Zali, Prevalence and typing of adenoviruses in stool of Iranian children with acute diarrhoea: a comparative study by molecular and serological assays, Clinical Microbiology & Infection, 2006, 12 (s4), P527.

• F. Motevali, M. Ghazanfari, M. Zali, F. Roohvand. PCR-based diagnosis of Enteric adenoviruses in Clinic. J. of Laboratory Diagnosis. (ISSN 1561-6363), 2006, No.43, p: 16-23, (WWW.TASHKHIS.COM) (in Persian with English abstract).

• F. Motevali, M. Ghazanfari, S. Salmanzadeh-Ahrabi, F. Jafari, M. Zali, Prevalence and typing of adenoviruses in stool of Iranian children with acute diarrhoea: a comparative study by molecular and serological assays, F. Roohvand, Clinical Microbiology & Infection, 2006, 12 (s4), P527.